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Goat anti-Human IgE Antibody (Alkaline Phosphatase (AP))

WB, EIA Polyclonal IgG Alkaline Phosphatase (AP)
Catalog No. ABIN318515

Quick Overview for Goat anti-Human IgE Antibody (Alkaline Phosphatase (AP)) (ABIN318515)

Target

IgE

Clonality

  • 70
  • 36
Polyclonal

Conjugate

  • 37
  • 19
  • 17
  • 17
  • 6
  • 6
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Alkaline Phosphatase (AP)

Application

  • 63
  • 50
  • 36
  • 28
  • 20
  • 17
  • 15
  • 14
  • 10
  • 8
  • 8
  • 6
  • 5
  • 3
  • 3
  • 3
  • 1
  • 1
  • 1
Western Blotting (WB), Enzyme Immunoassay (EIA)

Isotype

IgG
  • Reactivity

    • 50
    • 31
    • 15
    • 9
    • 6
    • 5
    • 3
    • 2
    • 1
    • 1
    Human, Monkey

    Host

    • 58
    • 25
    • 16
    • 11
    • 4
    • 1
    Goat

    Cross-Reactivity

    Monkey

    Purification

    Purified IgG prepared by affinity chromatography
  • Application Notes

    ELISA: 1/1000-1/10000. Western Blot: 1/1000-1/5000.

    Other applications not tested.
    Otimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Concentration

    0.5 mg/mL

    Buffer

    50 mM HEPES containing 0.1 M NaCl, 1 mM MgCl2, 0.1 mM ZnCl2 with 0.09 % Sodium Azide as preservative and 0.2 % BSA as stabilizer.

    Preservative

    Sodium azide

    Precaution of Use

    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

    Handling Advice

    Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
    Do not add Sodium azide.
    Dilute only prior to immediate use
    Each reagent is stable for the period shown on the bottle label if stored as directed.

    Storage

    4 °C

    Storage Comment

    Store the antibody undiluted at 2-8 °C.
  • Target

    IgE

    Background

    Synonyms: Immunoglobulin E human
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