Donkey anti-Mouse IgG Antibody (HRP)

Catalog No. ABIN336465

Quick Overview for Donkey anti-Mouse IgG Antibody (HRP) (ABIN336465)

Target: IgG

Clonality: Polyclonal

Conjugate: HRP

Product Details

Reactivity: Mouse

Host: Donkey

Purpose: Donkey anti-Mouse IgG (H&L) - Affinity Pure, HRP Conjugate

Specificity: Based on IEP, this antibody reacts with: • heavy (i³) chains on mouse IgG • light chains on all mouse immunoglobulins

Cross-Reactivity (Details): Based on IEP, no reactivity is observed to: • non-immunoglobulin mouse serum immunoglobulins

Purification: Affinity purified using solid phase Mouse IgG

Purity: Affinity purified antibody is > 95 % based on SDS-PAGE. E 1 % at 280 nm = 13.0

Immunogen: Purified Mouse IgG, whole molecule

Application Details

Comment:

HRP: Enzyme derived from the plant horseradish, A small molecule that does not cause steric hindrance in antibody-antigen reactions, Highly sensitive, can detect low levels of antibody and can be used in a sensitive ECL assay, Hydrogen peroxide oxidizes chromogenic substrates such as TMB, ABTS, and OPD to produce a soluble product with a distinct color which can be measured by spectrophotometer, The reaction product has a high color stability after the reaction is stopped. Detection OD of 450 nm

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Rehydrate with 1.1 mL of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.

Concentration: 1.0 mg/mL

Buffer: PBS, 1 % BSA & 0.1 % proclin 150, contains 0.1 % (v/v) Proclin 150

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Store lyophilized material at 2-8 °C. For long term storage after reconstitution, dilute with 50 % glycerol and store at -20 °C as a liquid.

Storage: 4 °C

Storage Comment: Store freeze-dried powder at 2-8 °C.

Target Details

Target: IgG

Target Type: Antibody