Goat anti-Chicken IgY Antibody (Alkaline Phosphatase (AP))

Catalog No. ABIN336518

Quick Overview for Goat anti-Chicken IgY Antibody (Alkaline Phosphatase (AP)) (ABIN336518)

Target: IgY

Clonality: Polyclonal

Conjugate: Alkaline Phosphatase (AP)

Product Details

Reactivity: Chicken

Host: Goat

Purpose: Goat anti-Chicken IgY (H&L) - Affinity Pure, ALP Conjugate

Specificity: Based on IEP, this antibody reacts with: • heavy (i³) chains on chicken IgG/Y • light chains on all chicken immunoglobulins

Cross-Reactivity (Details): Based on IEP, no reactivity is observed to: • non-immunoglobulin chicken serum immunoglobulins

Purification: Affinity purified using solid phase Chicken IgG/Y

Purity: Affinity purified antibody is > 95 % based on SDS-PAGE. E 1 % at 280 nm = 13.0

Immunogen: Purified Chicken IgG/Y, whole molecule

Application Details

Comment:

ALP: Enzyme derived from calf intestine (U.S. origin), Catalyzes the hydrolysis of phosphate groups from a substrate, which produces a colored reaction product or a release of light, The most common substrate for ALP is pNPP, which produces a soluble product, Unaffected by biological components in the ELISA assay, and produces consistent results, The reaction rate of ALP is linear, so its detection sensitivity can be increased by increasing the length of the reaction time. Detection OD of 405 nm

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.5 mg/mL

Buffer: 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, contains 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: 1 year from date of receipt. Prepare working dilution prior to use and then discard.

Storage: 4 °C,-20 °C

Storage Comment: Store undiluted liquid at 2-8 °C. For storage at -20 °C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity.

Expiry Date: 12 months

Target Details

Target: IgY

Target Type: Antibody