Goat anti-Human IgM Antibody (Alkaline Phosphatase (AP))

Catalog No. ABIN336623

Quick Overview for Goat anti-Human IgM Antibody (Alkaline Phosphatase (AP)) (ABIN336623)

Target: IgM

Clonality: Polyclonal

Conjugate: Alkaline Phosphatase (AP)

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC), Immunoassay (IA)

Product Details

Reactivity: Human

Host: Goat

Purpose: Goat anti-Human IgM ( μ chain) - Affinity Pure, ALP Conjugate, min x w/human IgA + IgG

Specificity: Based on IEP, this antibody reacts with: • heavy ( μ) chains on human IgM

Cross-Reactivity (Details): Based on IEP, no reactivity is observed to: • non-immunoglobulin human serum proteins • light chains on all human immunoglobulins • human IgA or IgG

Purification: Affinity purified using solid phase Human IgM

Purity: > 95 % based on SDS-PAGE. E 1 % at 280 nm = 13.0

Immunogen: Purified Human IgM, mu chain

Application Details

Application Notes: This conjugate is suitable for all immunoassay applications.
The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1:500-1:2,000 for ELISA/Western blot 1:20. 1:2,000 for Immunohistochemistry 1:50. 1:5,000 for Immunocytochemistry

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.5 mg/mL

Buffer: 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, contains 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: 1 year from date of receipt. Prepare working dilution prior to use and then discard.

Storage: 4 °C,-20 °C

Storage Comment: Store undiluted liquid at 2-8 °C. For storage at -20 °C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard.

Expiry Date: 12 months

Target Details

Target: IgM

Target Type: Antibody