Rabbit anti-Mouse IgG Antibody (HRP)

Catalog No. ABIN336829

Quick Overview for Rabbit anti-Mouse IgG Antibody (HRP) (ABIN336829)

Target: IgG

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunoassay (IA)

Product Details

Reactivity: Mouse

Host: Rabbit

Purification: Affinity purified using solid phase Mouse IgG
May contain small amounts of intact IgG

Purity: > 90 % based on SDS-PAGE

Application Details

Application Notes: This conjugate is suitable for all immunoassay applications.
The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1:500-1:5,000 for Immunohistochemistry 1:10,000-1:50,000 for ELISA/Western blot

Comment:

HRP: Enzyme derived from the plant horseradish, A small molecule that does not cause steric hindrance in antibody-antigen reactions, Highly sensitive, can detect low levels of antibody and can be used in a sensitive ECL assay, Hydrogen peroxide oxidizes chromogenic substrates such as TMB, ABTS, and OPD to produce a soluble product with a distinct color which can be measured by spectrophotometer, The reaction product has a high color stability after the reaction is stopped. Detection OD of 450 nm
Country of Origin: Rabbit antiserum was obtained from animals of US origin and under the care of a registered veterinarian.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. Preservative: 0.1 % (v/v) Kathon CG

Preservative: Kathon CG

Handling Advice: Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: 4 °C/-20 °C

Target Details

Target: IgG

Target Type: Antibody