Rabbit anti-Human Ig (Chain lambda) Antibody

Catalog No. ABIN336981

Quick Overview for Rabbit anti-Human Ig (Chain lambda) Antibody (ABIN336981)

Target: Ig

Clonality: Polyclonal

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details

Binding Specificity: Chain lambda

Reactivity: Human, Monkey

Host: Rabbit

Specificity: Molecular Weight: 25 kDa.

Immunogen: Purified human Lambda light chain from human Bence Jones proteins

Application Details

Application Notes: IHC, ICC (frozen or formalin–fixed paraffin-embedded (FFPE) tissue sections, cell smears) 1:50-1:200 using streptavidin-Biotin system or polymer system. Prediluted antibody is used directly without dilution. FFPE tissue sections require antigen retriever (boiling tissue in 10 mM citrate pH 6.0 for 10-20 minutes, followed by cooling at RT for 15-20 minutes). The optimum dilution should be determined by the individual lab. Positive Control: Human tonsil. Cellular Localization: Cytoplasmic.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibody is supplied with 1% BSA in PBS with 0.05% azide, pH 7.4. The Prediluted antibody is supplied in our Universal antibody dilution buffer (AR-6526) green in color.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details

Target: Ig

Alternative Name: Immunoglobulin Ig

Background: Human Lambda chain is a 25 kDa light chain present on all 5 immunoglobulin’s (IgA, IgG, IgM, IgD and IgE )and is also present in free form. Kappa and Lambda light chains consists of a variable region and a constant region and can be easily be differentiated by the antigenic properties of a constant region. The ratio of Kappa to Lambda chain is 70:30, most of them are bound to heavy chains of immunoglobulin. This antibody is useful in identification of leukemias, plasmasacytomas and certain non-Hodgkin’s lymphomas. In normal human serum lambda chains are present 0.4-4.2mg/ml. In multiple myeloma or B-cell malignancies these levels are increased. Higher amounts are also present in Bence Jones patient’s urine.