Goat anti-Mouse IgM Antibody (APC) - Preadsorbed

Catalog No. ABIN375765

Quick Overview for Goat anti-Mouse IgM Antibody (APC) - Preadsorbed (ABIN375765)

Target: IgM

Clonality: Polyclonal

Conjugate: APC

Application: ELISA, Flow Cytometry (FACS)

Isotype: IgG

Product Details

Reactivity: Mouse

Host: Goat

Specificity: Reacts with the heavy chain of mouse IgM

Characteristics: Goat Anti-Mouse IgM, Human ads-APC

Purification:

Purification Method: Affinity chromatography on mouse IgM covalently linked to agarose.

Preadsorption: Human Adsorbed

Application Details

Application Notes:

• Applications: Quality tested applications include - ELISA , FLISA FC ,
• Other referenced applications include - ELISPOT , IHC-FS , IHC-PS , ICC , EM , WB , IP , Purification , Stim
• Working Dilutions: ELISA AP conjugate 1:2,000 - 1:4,000 HRP conjugate 1:4,000 - 1:8,000 BGAL conjugate 1:500 BIOT conjugate 1:5,000 - 1:20,000 FLISA FITC, TRITC, TXRD, AF488, and AF555 conjugates 1:100 - 1:400 PE, APC, CY5, and AF647 conjugates 1 g/mL Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE, APC, CY5, PE/CY7, and AF647 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

Excitation/Emission wavelength: 650 nm/660 nm

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg in 1.0 mL or 0.25 mg in 0.5 mL of PBS/Sodium azide and a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References

Howie, Delaney, Wang, Er, Vidarsson, Stegmann, Kapp, Lebedev, Wu, AuBuchon, Zimring: "Serological blind spots for variants of human IgG3 and IgG4 by a commonly used anti-immunoglobulin reagent." in: Transfusion, Vol. 56, Issue 12, pp. 2953-2962, (2017) (PubMed).

Target Details

Target: IgM

Target Type: Antibody