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Goat anti-Mouse IgG2a Antibody (APC) - Preadsorbed

ELISA, FACS Polyclonal IgG APC
Catalog No. ABIN375770

Quick Overview for Goat anti-Mouse IgG2a Antibody (APC) - Preadsorbed (ABIN375770)

Target

IgG2a

Clonality

  • 70
  • 32
Polyclonal

Conjugate

  • 27
  • 14
  • 13
  • 12
  • 9
  • 5
  • 5
  • 5
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
APC

Application

  • 59
  • 45
  • 43
  • 39
  • 23
  • 19
  • 17
  • 12
  • 10
  • 10
  • 5
  • 4
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
  • 1
ELISA, Flow Cytometry (FACS)

Isotype

IgG
  • Reactivity

    • 99
    • 21
    • 1
    Mouse

    Host

    • 53
    • 31
    • 17
    • 15
    • 5
    Goat

    Specificity

    Reacts with the heavy chain of mouse IgG2a

    Characteristics

    Goat Anti-Mouse IgG2a, Human ads-APC

    Purification

    Purification Method: Affinity chromatography on mouse IgG2a covalently linked to agarose.

    Preadsorption: Human Adsorbed

  • Application Notes

    • Applications: Quality tested applications include - ELISA , FLISA FC ,
    • Other referenced applications include - ELISPOT , IHC-FS , IHC-PS , ICC , EM , WB , SPR , Purification
    • Working Dilutions: ELISA AP conjugate 1:2,000 - 1:4,000 HRP conjugate 1:4,000 - 1:8,000 BIOT conjugate 1:5,000 - 1:20,000 FLISA FITC, TRITC, TXRD, AF488, and AF555 conjugates 1:100 - 1:400 PE, APC, CY5, and AF647 conjugates 1 g/mL Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE, PE/TXRD, APC, CY5, PE/CY7, APC/CY7, and AF647 0.1 g/106 cells conjugates For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

    Comment

    Excitation/Emission wavelength: 650 nm/660 nm

    Restrictions

    For Research Use only
  • Buffer

    0.25 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Do not freeze!
    Protect conjugated products from light.

    Each reagent is stable for the period shown on the bottle label if stored as directed.

    Storage

    4 °C

    Storage Comment

    Store at 2-8°C
  • Gao, Lowen, Wang, Palese: "A nine-segmented influenza A virus carrying H1 and H3 subtype hemagglutinins." in: Journal of virology, (2010) (PubMed).

  • Target

    IgG2a

    Target Type

    Antibody
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