Goat anti-Rabbit IgG (Heavy & Light Chain) Antibody (APC) - Preadsorbed

Catalog No. ABIN375800

Quick Overview for Goat anti-Rabbit IgG (Heavy & Light Chain) Antibody (APC) - Preadsorbed (ABIN375800)

Target: IgG

Clonality: Polyclonal

Conjugate: APC

Application: ELISA, Flow Cytometry (FACS)

Isotype: IgG

Product Details

Binding Specificity: Heavy & Light Chain

Reactivity: Rabbit

Host: Goat

Specificity: Reacts with the heavy and light chains of rabbit IgG and the light chains of rabbit IgM

Characteristics: Goat Anti-Rabbit IgG(H+L), Mouse/Human ads-APC

Purification:

Purification Method: Affinity chromatography on pooled rabbit IgG covalently linked to agarose.

Preadsorption: Mouse/Human adsorbed

Application Details

Application Notes:

• Applications: Quality tested applications include - ELISA , FLISA FC ,
• Other referenced applications include - IHC-FS , IHC-PS , IHC-WM , ICC , WB , Sep
• Working Dilutions: ELISA AP conjugate 1:2,000 - 1:4,000 HRP conjugate 1:4,000 - 1:8,000 BIOT conjugate 1:5,000 - 1:20,000 FLISA FITC, TRITC, TXRD, AF488, and AF555 conjugates 1:100 - 1:400 PE, APC, and AF647 conjugates ≤ 1 μg/mL Flow Cytometry FITC, BIOT, and AF488 conjugates ≤ 1 μg/106 cells PE, APC, SPRD, and AF647 conjugates ≤ 0.1 μg/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 μL

Comment:

Excitation/Emission wavelength: 650 nm/660 nm

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg in 1.0 mL or 0.25 mg in 0.5 mL of PBS/Sodium azide and a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References

Vecchio, Golino, Pisano, Albano, Falcone, Ceglia, Iaccino, Mimmi, Fiume, Giurato, Britti, Scala, Quinto: "IBTK contributes to B-cell lymphomagenesis in Eμ-myc transgenic mice conferring resistance to apoptosis." in: Cell death & disease, Vol. 10, Issue 4, pp. 320, (2019) (PubMed).

Wu, Deng, Wang, Zhao, Wang, Rao, Xu, Zhou, Choi, Rizvi, Remke, Rubin, Johnson, Carroll, Stemmer-Rachamimov, Wu, Zheng, Xin, Ratner, Lu: "Programming of Schwann Cells by Lats1/2-TAZ/YAP Signaling Drives Malignant Peripheral Nerve Sheath Tumorigenesis." in: Cancer cell, Vol. 33, Issue 2, pp. 292-308.e7, (2018) (PubMed).

Albano, Chiurazzi, Mimmi, Vecchio, Pastore, Cimmino, Frieri, Iaccino, Pisano, Golino, Fiume, Mallardo, Scala, Quinto: "The expression of inhibitor of bruton's tyrosine kinase gene is progressively up regulated in the clinical course of chronic lymphocytic leukaemia conferring resistance to apoptosis." in: Cell death & disease, Vol. 9, Issue 1, pp. 13, (2018) (PubMed).

Target Details

Target: IgG

Target Type: Antibody