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Goat anti-Mouse Ig Antibody (Biotin) - Preadsorbed

ELISA, FACS Polyclonal IgG Biotin F(ab')2 fragment
Catalog No. ABIN375905

Quick Overview for Goat anti-Mouse Ig Antibody (Biotin) - Preadsorbed (ABIN375905)

Target

Ig

Fragment

F(ab')2 fragment

Clonality

  • 112
  • 95
Polyclonal

Conjugate

  • 52
  • 24
  • 23
  • 21
  • 17
  • 14
  • 11
  • 11
  • 5
  • 4
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Biotin

Application

  • 171
  • 136
  • 99
  • 67
  • 56
  • 26
  • 22
  • 12
  • 11
  • 7
  • 7
  • 6
  • 4
  • 2
  • 2
  • 1
  • 1
ELISA, Flow Cytometry (FACS)

Isotype

IgG
  • Reactivity

    • 116
    • 61
    • 20
    • 17
    • 9
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Mouse

    Host

    • 101
    • 77
    • 34
    • 18
    • 2
    • 1
    • 1
    Goat

    Specificity

    Reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b, IgG2c, IgG3, IgM, and IgA

    Characteristics

    Goat F(ab')2 Anti-Mouse Ig, Human ads-BIOT

    Purification

    Preadsorption: Human adsorbed

  • Application Notes

    • Applications: Quality tested applications include - ELISA , FLISA FC ,
    • Other referenced applications include - ELISPOT , ICC , WB , Stim
    • Working Dilutions: ELISA AP conjugate 1:2,000 - 1:4,000 HRP conjugate 1:4,000 - 1:8,000 BIOT conjugate 1:5,000 - 1:20,000 FLISA FITC, AF488, and AF555 conjugates 1:100 - 1:400 PE, APC, CY5, and AF647 conjugates 1 g/mL Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE, APC, SPRD, CY5, PE/CY5.5, and AF647 conjugates 0.1 g/106 cells PE/TXRD 0.03 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

    Restrictions

    For Research Use only
  • Concentration

    0.5 mg/mL

    Buffer

    0.5 mg in 1.0 mL of PBS/Sodium azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Avoid cycles of freezing and thawing.

    Storage

    4 °C

    Storage Comment

    Store at 2-8°C
  • Target

    Ig
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