Goat anti-Mouse IgG (Heavy & Light Chain) Antibody (Biotin) - Preadsorbed

Catalog No. ABIN375911

Quick Overview for Goat anti-Mouse IgG (Heavy & Light Chain) Antibody (Biotin) - Preadsorbed (ABIN375911)

Target: IgG

Fragment: F(ab')2 fragment

Clonality: Polyclonal

Conjugate: Biotin

Application: ELISA, Flow Cytometry (FACS)

Isotype: IgG

Product Details

Binding Specificity: Heavy & Light Chain

Reactivity: Mouse

Host: Goat

Specificity: Reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b, IgG2c, and IgG3 and with the light chains of mouse IgM and IgA

Characteristics: Goat F(ab')2 Anti-Mouse IgG(H+L), Human ads-BIOT

Purification:

Preadsorption: Human adsorbed

Application Details

Application Notes:

• Applications: Quality tested applications include - ELISA , FLISA FC ,
• Other referenced applications include - ELISPOT , IHC-FS , IHC-PS , ICC , WB
• Working Dilutions: ELISA AP conjugate 1:2,000 - 1:4,000 HRP conjugate 1:4,000 - 1:8,000 BIOT conjugate 1:5,000 - 1:20,000 FLISA FITC and AF488 conjugates 1:100 - 1:400 AF555 conjugate 1:50 - 1:200 PE, APC, and CY5 conjugates 1 g/mL Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE, APC, SPRD, and CY5 conjugates 0.1 g/106 cells PE/CY5.5 conjugate 0.05 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg in 1.0 mL of PBS/Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid cycles of freezing and thawing.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details

Target: IgG

Target Type: Antibody