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Goat anti-Mouse IgG2a (Heavy Chain) Antibody (Biotin)

ELISA, FACS, IHC, WB Polyclonal IgG Biotin
Catalog No. ABIN375919

Quick Overview for Goat anti-Mouse IgG2a (Heavy Chain) Antibody (Biotin) (ABIN375919)

Target

IgG2a

Clonality

  • 70
  • 32
Polyclonal

Conjugate

  • 27
  • 13
  • 13
  • 12
  • 9
  • 5
  • 5
  • 5
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Biotin

Application

  • 59
  • 44
  • 43
  • 38
  • 23
  • 19
  • 17
  • 12
  • 10
  • 10
  • 5
  • 4
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
  • 1
ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)

Isotype

IgG
  • Binding Specificity

    • 40
    • 9
    • 7
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    Heavy Chain

    Reactivity

    • 99
    • 21
    • 1
    Mouse

    Host

    • 53
    • 31
    • 17
    • 15
    • 5
    Goat

    Specificity

    Reacts with the heavy chain of mouse IgG 2a as demonstrated by ELISA and flow cytometry.

    Characteristics

    Source: Pooled antisera from goats hyperimmunized with mouse IgG 2a paraproteins.
    To insure lot-to-lot consistency, each batch of product is tested by ELISA, PCFIA and/or flow cytometry for conformance with characteristics of a standard reference reagent.

    Purification

    Affinity chromatography on mouse IgG 3 covalently linked to agarose.
  • Application Notes

    Working Dilution:
    <= 1 µg/10^6 cells or 1:5,000-1:20,000
    Representative data are included in this product insert.

    Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

    Restrictions

    For Research Use only
  • Handling Advice

    Avoid cycles of freezing and thawing.

    Storage

    4 °C
  • Target

    IgG2a

    Target Type

    Antibody
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