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Goat anti-Human Ig (Heavy & Light Chain) Antibody (Biotin)

ELISA, FACS, WB Polyclonal IgG Biotin
Catalog No. ABIN375946

Quick Overview for Goat anti-Human Ig (Heavy & Light Chain) Antibody (Biotin) (ABIN375946)

Target

Ig

Clonality

  • 112
  • 95
Polyclonal

Conjugate

  • 52
  • 24
  • 23
  • 21
  • 17
  • 14
  • 11
  • 11
  • 5
  • 4
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Biotin

Application

  • 171
  • 136
  • 99
  • 66
  • 56
  • 26
  • 22
  • 12
  • 11
  • 7
  • 7
  • 6
  • 4
  • 2
  • 2
  • 1
  • 1
ELISA, Flow Cytometry (FACS), Western Blotting (WB)

Isotype

IgG
  • Binding Specificity

    • 82
    • 77
    • 64
    • 31
    • 4
    • 4
    • 2
    • 1
    Heavy & Light Chain

    Reactivity

    • 115
    • 62
    • 20
    • 17
    • 9
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human

    Host

    • 101
    • 77
    • 34
    • 18
    • 2
    • 1
    • 1
    Goat

    Specificity

    Reacts with the heavy and light chains of human IgM, IgG and IgA, and with the light chains of human IgD as demonstrated by ELISA and flow cytometry.

    Characteristics

    Source: Pooled antisera from goats hyperimmunized with normal human IgM and IgG, and human IgM, IgG and IgA paraproteins.
    To insure lot-to-lot consistency, each batch of product is tested by ELISA, PCFIA and/or flow cytometry for conformance to characteristics of a standard reference reagent.

    Purification

    Affinity chromatography on pooled human immunoglobulin covalently linked to agarose.
  • Application Notes

    Working Dilution:
    <= 1 µg/10^6 cells or 1:5,000-1:20,000
    Representative data are included in this product insert.

    Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

    Restrictions

    For Research Use only
  • Handling Advice

    Avoid cycles of freezing and thawing.

    Storage

    4 °C
  • Fan, Longnecker: "The Ig-like v-type domain of paired Ig-like type 2 receptor alpha is critical for herpes simplex virus type 1-mediated membrane fusion." in: Journal of virology, Vol. 84, Issue 17, pp. 8664-72, (2010) (PubMed).

  • Target

    Ig
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