Goat anti-Human Immunoglobulin kappa Chain Complex (Igk) (Chain kappa) Antibody (Biotin)

Catalog No. ABIN375960

Quick Overview for Goat anti-Human Immunoglobulin kappa Chain Complex (Igk) (Chain kappa) Antibody (Biotin) (ABIN375960)

Target: Igk (Immunoglobulin kappa Chain Complex (Igk))

Fragment: F(ab')2 fragment

Clonality: Polyclonal

Conjugate: Biotin

Application: Flow Cytometry (FACS), ELISA

Isotype: IgG

Product Details

Binding Specificity: Chain kappa

Reactivity: Human

Host: Goat

Specificity: Reacts with human λ light chains as demonstrated by ELISA and flow cytometry.

Cross-Reactivity (Details): Cross Absorption: Mouse IgM, IgG 1, IgG 2a, IgG 3 and IgA, pooled human sera and purified human paraproteins.

Characteristics: Goat F(ab')2 Anti-Human Kappa-BIOT

Purification: Gel filtration chromatography of pepsin digested antibody.

Application Details

Application Notes:

• Applications: Quality tested applications include - ELISA , FLISA FC ,
• Other referenced applications include - ICC , WB , SPR , Stim
• Working Dilutions: ELISA HRP conjugate 1:4,000 - 1:8,000 BIOT conjugate 1:5,000 - 1:20,000 FLISA FITC and AF488 conjugates 1:200 - 1:400 AF555 conjugate 1:100 - 1:400 PE and AF647 conjugates 1 g/mL Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells AF647 conjugate 0.1 g/106 cells PE conjugate 0.05 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

B cell enumeration

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg in 1.0 mL of PBS/Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details

Target: Igk (Immunoglobulin kappa Chain Complex (Igk))

Alternative Name: Kappa