Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Mouse anti-Human IgG (Fc Region) Antibody (Cy5)

ELISA, FACS Monoclonal H2 IgG2b Cy5
Catalog No. ABIN376064

Quick Overview for Mouse anti-Human IgG (Fc Region) Antibody (Cy5) (ABIN376064)

Target

IgG

Clonality

  • 3438
  • 229
  • 16
  • 1
Monoclonal

Conjugate

  • 665
  • 422
  • 422
  • 398
  • 274
  • 262
  • 235
  • 118
  • 105
  • 72
  • 67
  • 64
  • 61
  • 58
  • 58
  • 50
  • 44
  • 43
  • 39
  • 39
  • 39
  • 37
  • 35
  • 35
  • 32
  • 31
  • 31
  • 30
  • 29
  • 29
  • 29
  • 29
  • 29
  • 28
  • 28
  • 28
  • 28
  • 28
  • 28
  • 28
  • 28
  • 27
  • 26
  • 26
  • 25
  • 21
  • 21
  • 19
  • 17
  • 17
  • 16
Cy5

Application

  • 2118
  • 2050
  • 1884
  • 1873
  • 999
  • 920
  • 896
  • 520
  • 424
  • 234
  • 234
  • 149
  • 102
  • 98
  • 88
  • 84
  • 51
  • 33
  • 30
  • 28
  • 25
  • 20
  • 15
  • 11
  • 9
  • 8
  • 7
  • 5
  • 5
  • 4
  • 4
  • 4
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
ELISA, Flow Cytometry (FACS)

Clone

H2

Isotype

IgG2b
  • Binding Specificity

    • 2397
    • 516
    • 417
    • 146
    • 130
    • 128
    • 66
    • 29
    • 28
    • 28
    • 21
    • 9
    • 5
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Fc Region

    Reactivity

    • 752
    • 703
    • 607
    • 468
    • 410
    • 244
    • 213
    • 176
    • 146
    • 141
    • 124
    • 110
    • 107
    • 101
    • 82
    • 62
    • 47
    • 28
    • 27
    • 16
    • 15
    • 10
    • 8
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human

    Host

    • 1773
    • 1583
    • 567
    • 281
    • 122
    • 112
    • 41
    • 32
    • 22
    • 21
    • 16
    • 11
    • 11
    Mouse

    Specificity

    Reacts with the Fc portion of the heavy chain of all subclasses of human IgG as demonstrated by ELISA

    Characteristics

    Mouse Anti-Human IgG Fc-CY5

    Purification

    Purified

    Immunogen

    Unknown
  • Application Notes

    • Applications: ELISA - Quality tested , FLISA - Quality tested FC - Quality tested , ELISPOT - Reported in literature , WB - Reported in literature , Multiplex - Reported in literature
    • Working Dilutions: ELISA AP conjugate 1:2,000 - 1:8,000 HRP conjugate 1:4,000 - 1:8,000 BIOT conjugate 1:10,000 - 1:20,000 FLISA FITC conjugate 1:200 - 1:600 AF488 and AF555 conjugates 1:100 - 1:400 PE and AF647 conjugates 1 g/mL Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC, BIOT, and AF488 conjugates 1 g/106 cells CY5 conjugate 0.3 g/106 cells PE, APC, SPRD, and AF647 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

    Comment

    Excitation / Emission wavelength: 652 nm / 672 nm

    Restrictions

    For Research Use only
  • Concentration

    0.5 mg/mL

    Buffer

    0.1 mg in 0.2 mL of PBS/Sodium azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Protect conjugated products from light.
    Each reagent is stable for the period shown on the bottle label if stored as directed.

    Storage

    4 °C

    Storage Comment

    Store at 2-8°C
  • Target

    IgG

    Target Type

    Antibody
You are here:
Chat with us!