Goat anti-Mouse IgG2a (Heavy Chain) Antibody (HRP)

Catalog No. ABIN376250

Quick Overview for Goat anti-Mouse IgG2a (Heavy Chain) Antibody (HRP) (ABIN376250)

Target: IgG2a

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Product Details

Binding Specificity: Heavy Chain

Reactivity: Mouse

Host: Goat

Specificity: Reacts with the heavy chain of mouse IgG 2a as demonstrated by ELISA and flow cytometry.

Characteristics: Source: Pooled antisera from goats hyperimmunized with mouse IgG 2a paraproteins.

Purification: Affinity chromatography on mouse IgG 2a covalently linked to agarose.

Application Details

Application Notes: Working Dilution:
ELISA: 1:4.000 - 1:8.000
Immunofluorescent Staining Amount Used: < 1 µg/10^6 cells
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Restrictions: For Research Use only

Handling

Format: Liquid

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: 4 °C

References

Staszewski, Reece, ODonnell, Cunningham: "Drug treatment of malaria infections can reduce levels of protection transferred to offspring via maternal immunity." in: Proceedings. Biological sciences / The Royal Society, (2012) (PubMed).

Yu, Vajdy: "A novel retinoic acid, catechin hydrate and mustard oil-based emulsion for enhanced cytokine and antibody responses against multiple strains of HIV-1 following mucosal and systemic vaccinations." in: Vaccine, Vol. 29, Issue 13, pp. 2429-36, (2011) (PubMed).

Stegmann, Kamphuis, Meijerhof, Goud, de Haan, Wilschut: "Lipopeptide-adjuvanted respiratory syncytial virus virosomes: A safe and immunogenic non-replicating vaccine formulation." in: Vaccine, (2010) (PubMed).

Target Details

Target: IgG2a

Target Type: Antibody