Goat anti-Mouse IgE (Heavy Chain) Antibody (HRP)

Catalog No. ABIN376253

Quick Overview for Goat anti-Mouse IgE (Heavy Chain) Antibody (HRP) (ABIN376253)

Target: IgE

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Isotype: IgG

Product Details

Binding Specificity: Heavy Chain

Reactivity: Mouse

Host: Goat

Specificity: Reacts with the heavy chain of BALB/c mouse IgE as demonstrated by ELISA.

Characteristics: To insure lot-to-lot consistency, each batch of product is tested by ELISA for conformance to characteristics of a standard reference reagent. This product is not tested for reactivity to other species IgE.
Source: Pooled antisera from goats hyperimmunized with BALB/c mouse IgE paraproteins.

Application Details

Application Notes: Working Dilution:
Enzyme-Linked-Immunosorbent-Assay (ELISA) 1:4,000-1:8,000
Indirect immunostaining in conjunction with mouse IgE monoclonal antibodies.
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Restrictions: For Research Use only

Handling

Format: Liquid

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C or long-term at -20 °C.

References

Joshi, Adamcakova-Dodd, Jing, Wongrakpanich, Gibson-Corley, Thorne, Salem: "Development of a Poly (lactic-co-glycolic acid) Particle Vaccine to Protect Against House Dust Mite Induced Allergy." in: The AAPS journal, (2014) (PubMed).

Target Details

Target: IgE