Goat anti-Human Ig (Heavy & Light Chain) Antibody (HRP)

Catalog No. ABIN376265

Quick Overview for Goat anti-Human Ig (Heavy & Light Chain) Antibody (HRP) (ABIN376265)

Target: Ig

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Western Blotting (WB)

Isotype: IgG

Product Details

Binding Specificity: Heavy & Light Chain

Reactivity: Human

Host: Goat

Specificity: Reacts with the heavy and light chains of human IgM, IgG and IgA, and with the light chains of human IgD as demonstrated by ELISA and flow cytometry.

Characteristics: Source: Pooled antisera from goats hyperimmunized with normal human IgM and IgG, and human IgM, IgG and IgA paraproteins.
To insure lot-to-lot consistency, each batch of product is tested by ELISA, PCFIA and/or flow cytometry for conformance to characteristics of a standard reference reagent.

Purification: Affinity chromatography on pooled human immunoglobulin covalently linked to agarose.

Application Details

Application Notes: Working Dilution:
1:4,000-1:8,000
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Restrictions: For Research Use only

Handling

Format: Liquid

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: 4 °C

References

Perez, Vargis, Russ, Haselton, Wright: "Detection of Respiratory Syncytial Virus Using Nanoparticle Amplified Immuno-PCR." in: Analytical biochemistry, (2010) (PubMed).

Target Details

Target: Ig