Goat anti-Mouse IgG2b Antibody (PE) - Preadsorbed

Catalog No. ABIN376455

Quick Overview for Goat anti-Mouse IgG2b Antibody (PE) - Preadsorbed (ABIN376455)

Target: IgG2b

Clonality: Polyclonal

Conjugate: PE

Application: ELISA, Flow Cytometry (FACS)

Isotype: IgG

Product Details

Reactivity: Mouse

Host: Goat

Specificity: Reacts with the heavy chain of mouse IgG2b

Characteristics: Goat Anti-Mouse IgG2b, Human ads-PE

Purification:

Purification Method: Affinity chromatography on mouse IgG2b covalently linked to agarose.

Preadsorption: Human Adsorbed

Application Details

Application Notes:

• Applications: Quality tested applications include - ELISA , FLISA FC ,
• Other referenced applications include - ELISPOT , IHC-FS , IHC-PS , ICC , WB
• Working Dilutions: ELISA AP conjugate 1:2,000 - 1:4,000 HRP conjugate 1:4,000 - 1:8,000 BIOT conjugate 1:5,000 - 1:20,000 FLISA FITC, TRITC, TXRD, AF488, and AF555 conjugates 1:100 - 1:400 PE, APC, CY5, and AF647 conjugates 1 g/mL Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE, APC, SPRD, CY5, PE/CY7, APC/CY7, and AF647 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Restrictions: For Research Use only

Handling

Buffer: 0.25 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References

Li, Irrazabal, So, Berru, Du, Lam, Ling, Gommerman, Pan-Hammarström, Martin: "The H2B deubiquitinase Usp22 promotes antibody class switch recombination by facilitating non-homologous end joining." in: Nature communications, Vol. 9, Issue 1, pp. 1006, (2018) (PubMed).

Paßlack, Kohn, Doherr, Zentek: "Impact of Dietary Protein Concentration and Quality on Immune Function of Cats." in: PLoS ONE, Vol. 12, Issue 1, pp. e0169822, (2017) (PubMed).

Eichin, Laurila, Jalkanen, Salmi: "CD73 Activity is Dispensable for the Polarization of M2 Macrophages." in: PLoS ONE, Vol. 10, Issue 8, pp. e0134721, (2016) (PubMed).

Tsai, Murakami, Gariepy: "Postnatal intestinal engraftment of prospectively selected enteric neural crest stem cells in a rat model of Hirschsprung disease." in: Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society, Vol. 23, Issue 4, pp. 362-9, (2011) (PubMed).

Target Details

Target: IgG2b

Target Type: Antibody