Rat anti-Mouse IgG2a Antibody (PE)

Catalog No. ABIN376466

Quick Overview for Rat anti-Mouse IgG2a Antibody (PE) (ABIN376466)

Target: IgG2a

Clonality: Monoclonal

Conjugate: PE

Application: ELISA, Flow Cytometry (FACS)

Clone: SB84a

Isotype: IgG1

Product Details

Reactivity: Mouse

Host: Rat

Specificity: Mouse IgG2a

Characteristics: Rat Anti-Mouse IgG2a-PE

Purification: Purified

Immunogen: Mouse IgG2a hybridoma

Application Details

Application Notes:

• Applications: FC - Quality tested , ELISA - Quality tested , FLISA - Quality tested IHC-FS - Reported in literature , ICC , WB , Multiplex - Reported in literature
• Working Dilutions: Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE conjugate 0.2 g/106 cells AF647 conjugate 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L ELISA AP conjugate 1:1,000 - 1:4,000 HRP conjugate 1:4,000 - 1:8,000 BIOT conjugate 1:5,000 - 1:20,000 FLISA FITC and AF488 conjugates 1:200 - 1:400 AF555 conjugate 1:50 - 1:200 PE and AF647 conjugates 1 g/mL

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/Sodium azide and a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References

Mandt, Song, Scharenberg, Sahni: "SLC41A1 Mg(2+) transport is regulated via Mg(2+)-dependent endosomal recycling through its N-terminal cytoplasmic domain." in: The Biochemical journal, Vol. 439, Issue 1, pp. 129-39, (2011) (PubMed).

Target Details

Target: IgG2a

Target Type: Antibody