Goat anti-Human IgD Antibody (PE)

Catalog No. ABIN376508

Quick Overview for Goat anti-Human IgD Antibody (PE) (ABIN376508)

Target: IgD

Clonality: Polyclonal

Conjugate: PE

Application: Flow Cytometry (FACS), ELISA

Isotype: IgG

Product Details

Reactivity: Human

Host: Goat

Specificity: Reacts with the heavy chain of human IgD

Characteristics: Goat Anti-Human IgD-PE

Purification: Purification Method: Affinity chromatography on human IgD covalently linked to agarose.

Application Details

Application Notes:

• Applications: Quality tested applications include - ELISA FLISA FC ,
• Other referenced applications include - IHC-FS , IHC-PS , Sep
• Working Dilutions: ELISA AP conjugate 1:2,000 - 1:4,000 HRP conjugate 1:4,000 - 1:8,000 BIOT conjugate 1:5,000 - 1:20,000 FLISA FITC, TRITC, TXRD, AF488, and AF555 conjugates 1:100 - 1:400 PE and AF647 conjugates 1 g/mL Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE and AF647 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References

Han, Jones, Nicely, Reed, Shen, Mansouri, Louder, Trama, Alam, Edwards, Bonsignori, Tomaras, Korber, Montefiori, Mascola, Seaman, Haynes, Saunders: "Difficult-to-neutralize global HIV-1 isolates are neutralized by antibodies targeting open envelope conformations." in: Nature communications, Vol. 10, Issue 1, pp. 2898, (2019) (PubMed).

Blasi, Negri, LaBranche, Alam, Baker, Brunner, Gladden, Michelini, Vandergrift, Wiehe, Parks, Shen, Bonsignori, Tomaras, Ferrari, Montefiori, Santra, Haynes, Moody, Cara, Klotman: "IDLV-HIV-1 Env vaccination in non-human primates induces affinity maturation of antigen-specific memory B cells." in: Communications biology, Vol. 1, pp. 134, (2018) (PubMed).

Williams, Zhang, Jiang, Nicely, Fera, Luo, Moody, Liao, Alam, Kepler, Ramesh, Wiehe, Holland, Bradley, Vandergrift, Saunders, Parks, Foulger, Xia, Bonsignori, Montefiori, Louder, Eaton, Santra et al.: "Initiation of HIV neutralizing B cell lineages with sequential envelope immunizations. ..." in: Nature communications, Vol. 8, Issue 1, pp. 1732, (2018) (PubMed).

Bradley, Pollara, Santra, Vandergrift, Pittala, Bailey-Kellogg, Shen, Parks, Goodman, Eaton, Balachandran, Mach, Saunders, Weiner, Scearce, Sutherland, Phogat, Tartaglia, Reed, Hu, Theis, Pinter et al.: "Pentavalent HIV-1 vaccine protects against simian-human immunodeficiency virus challenge. ..." in: Nature communications, Vol. 8, pp. 15711, (2018) (PubMed).

Hammarlund, Thomas, Amanna, Holden, Slayden, Park, Gao, Slifka: "Plasma cell survival in the absence of B cell memory." in: Nature communications, Vol. 8, Issue 1, pp. 1781, (2018) (PubMed).

Target Details

Target: IgD

Target Type: Antibody