Goat anti-Human Immunoglobulin kappa Chain Complex (Igk) Antibody (PE)

Catalog No. ABIN376515

Quick Overview for Goat anti-Human Immunoglobulin kappa Chain Complex (Igk) Antibody (PE) (ABIN376515)

Target: Igk (Immunoglobulin kappa Chain Complex (Igk))

Clonality: Polyclonal

Conjugate: PE

Application: Flow Cytometry (FACS), ELISA

Isotype: IgG

Product Details

Reactivity: Human

Host: Goat

Specificity: Reacts with human light chains

Cross-Reactivity (Details): Cross Absorption: Pooled mouse sera and pooled mouse plasmacytoma/hybridoma proteins.

Characteristics: Goat Anti-Human Kappa-PE

Purification: Purification Method: Affinity chromatography on human light chains covalently linked to agarose.

Application Details

Application Notes:

• Applications: Quality tested applications include - ELISA , FLISA FC ,
• Other referenced applications include - ELISPOT , IHC-PS , ICC , WB , IP , SPR , Purification , Stim
• Working Dilutions: ELISA AP conjugate 1:2,000 - 1:4,000 HRP conjugate 1:4,000 - 1:8,000 BGAL conjugate 1:500 BIOT conjugate 1:5,000 - 1:20,000 FLISA FITC, TRITC, TXRD, AF488, and AF555 conjugates 1:100 - 1:400 PE and AF647 conjugates 1 g/mL Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE and AF647 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References

Lai, Verma, Bailey-Kellogg, Ackerman: "Towards conformational fidelity of a quaternary HIV-1 epitope: computational design and directed evolution of a minimal V1V2 antigen." in: Protein engineering, design & selection : PEDS, Vol. 31, Issue 4, pp. 121-133, (2018) (PubMed).

Target Details

Target: Igk (Immunoglobulin kappa Chain Complex (Igk))

Alternative Name: Kappa