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Mouse anti-Rat IgG1 (Fc Region) Antibody (PE)

ELISA, FACS Monoclonal G11C5 IgG2b PE
Catalog No. ABIN376528

Quick Overview for Mouse anti-Rat IgG1 (Fc Region) Antibody (PE) (ABIN376528)

Target

IgG1

Clonality

  • 94
  • 51
Monoclonal

Conjugate

  • 44
  • 21
  • 20
  • 17
  • 10
  • 6
  • 6
  • 4
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
PE

Application

  • 85
  • 59
  • 50
  • 45
  • 32
  • 20
  • 19
  • 14
  • 14
  • 9
  • 9
  • 9
  • 8
  • 6
  • 5
  • 3
  • 2
  • 2
  • 1
ELISA, Flow Cytometry (FACS)

Clone

G11C5

Isotype

IgG2b
  • Binding Specificity

    • 41
    • 17
    • 9
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Fc Region

    Reactivity

    • 98
    • 22
    • 15
    • 9
    • 7
    • 5
    • 4
    • 4
    • 3
    • 1
    • 1
    Rat

    Host

    • 64
    • 40
    • 30
    • 15
    • 10
    • 6
    • 1
    Mouse

    Specificity

    Reacts with the gamma 1 heavy chain (Fc) of Rat IgG1,

    Characteristics

    Mouse Anti-Rat IgG1-PE

    Purification

    Purified

    Immunogen

    Rat IgG1 hybridoma
  • Application Notes

    • Applications: FC - Quality tested ELISA - Quality tested FLISA - Quality tested IHC-FS - Reported in literature , ELISPOT - Reported in literature
    • Working Dilutions: Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE, CY5, and AF647 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L ELISA AP conjugate 1:1,000 - 1:4,000 HRP conjugate 1:4,000 - 1:8,000 BIOT conjugate 1:5,000 - 1:10,000 FLISA FITC and AF488 conjugates 1:200 - 1:400 AF555 conjugate 1:100 - 1:400 PE, CY5, and AF647 conjugates 1 g/mL

    Restrictions

    For Research Use only
  • Concentration

    0.1 mg/mL

    Buffer

    0.1 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent, store at 2-8 °C. Do not freeze! The Cyanine 5 (CY5) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/Sodium azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Do not freeze!
    Protect conjugated products from light.

    Each reagent is stable for the period shown on the bottle label if stored as directed.

    Storage

    4 °C

    Storage Comment

    Store at 2-8°C
  • Target

    IgG1

    Target Type

    Antibody
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