Goat anti-Rabbit Ig Antibody (PE) - Preadsorbed

Catalog No. ABIN376534

Quick Overview for Goat anti-Rabbit Ig Antibody (PE) - Preadsorbed (ABIN376534)

Target: Ig

Clonality: Polyclonal

Conjugate: PE

Application: ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Product Details

Reactivity: Rabbit

Host: Goat

Specificity: Reacts with the heavy and light chains of rabbit IgM and IgG as demonstrated by ELISA, FLISA, and/or flow cytometry, minimal cross reactivity with human immunoglobulins, may react with other species

Characteristics: Goat Anti-Rabbit Ig, Human ads-PE

Purification:

Preadsorption: Human adsorbed

Application Details

Application Notes:

• Applications: Flow Cytometry Enzyme-Linked-Immunosorbent-Assay (ELISA) Fluorescent-Linked-Immunosorbent-Assay (FLISA) Immunoblotting Immunohistochemistry
• Working Dilutions: Flow Cytometry FITC and BIOT conjugates 1 g/106 cells PE and AF647 conjugates 0.1 g/106 cells FLISA TRITC, TXRD, and AF555 conjugates 1:100 - 1:400 PE and AF647 conjugates 1 g/mL ELISA AP conjugate 1:2,000 - 1:4,000 HRP conjugate 1:4,000 - 1:8,000 BGAL conjugate 1:500 - 1:2,000 BIOT conjugate 1:5,000 - 1:20,000

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg in 1.0 mL or 0.25 mg in 0.5 mL of PBS/Sodium azide and a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References

Wang, Hilchey, DeDiego, Perry, Hyrien, Nogales, Garigen, Amanat, Huertas, Krammer, Martinez-Sobrido, Topham, Treanor, Sangster, Zand: "Broad cross-reactive IgG responses elicited by adjuvanted vaccination with recombinant influenza hemagglutinin (rHA) in ferrets and mice." in: PLoS ONE, Vol. 13, Issue 4, pp. e0193680, (2018) (PubMed).

Target Details

Target: Ig