Rat anti-Mouse IgG2a Antibody

Catalog No. ABIN376878

Quick Overview for Rat anti-Mouse IgG2a Antibody (ABIN376878)

Target: IgG2a

Clonality: Monoclonal

Application: ELISA, Flow Cytometry (FACS)

Clone: SB84a

Isotype: IgG1

Product Details

Reactivity: Mouse

Host: Rat

Specificity: Mouse IgG2a

Characteristics: Rat Anti-Mouse IgG2a-UNLB

Purification: Purified

Immunogen: Mouse IgG2a hybridoma

Application Details

Application Notes:

• Applications: FC - Quality tested , ELISA - Quality tested , FLISA - Quality tested IHC-FS - Reported in literature , ICC , WB , Multiplex - Reported in literature
• Working Dilutions: Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE conjugate 0.2 g/106 cells AF647 conjugate 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L ELISA AP conjugate 1:1,000 - 1:4,000 HRP conjugate 1:4,000 - 1:8,000 BIOT conjugate 1:5,000 - 1:20,000 FLISA FITC and AF488 conjugates 1:200 - 1:400 AF555 conjugate 1:50 - 1:200 PE and AF647 conjugates 1 g/mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added

Preservative: Without preservative

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References

Brummelman, Helm, Hamstra, van der Ley, Boog, Han, van Els: "Modulation of the CD4(+) T cell response after acellular pertussis vaccination in the presence of TLR4 ligation." in: Vaccine, (2015) (PubMed).

Target Details

Target: IgG2a

Target Type: Antibody