Rabbit anti-Human IgG Antibody (Alkaline Phosphatase (AP))

Catalog No. ABIN400368

Quick Overview for Rabbit anti-Human IgG Antibody (Alkaline Phosphatase (AP)) (ABIN400368)

Target: IgG

Clonality: Polyclonal

Conjugate: Alkaline Phosphatase (AP)

Application: Immunoassay (IA)

Product Details

Reactivity: Human

Host: Rabbit

Purification: Immunoaffinity chromatography

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

ALP: Enzyme derived from calf intestine (U.S. origin), Catalyzes the hydrolysis of phosphate groups from a substrate, which produces a colored reaction product or a release of light, The most common substrate for ALP is pNPP, which produces a soluble product, Unaffected by biological components in the ELISA assay, and produces consistent results, The reaction rate of ALP is linear, so its detection sensitivity can be increased by increasing the length of the reaction time. Detection OD of 405 nm

Restrictions: For Research Use only

Handling

Handling Advice: Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Target Details

Target: IgG

Target Type: Antibody