Mouse anti-Pig IgM Antibody

Catalog No. ABIN400639

Quick Overview for Mouse anti-Pig IgM Antibody (ABIN400639)

Target: IgM

Clonality: Monoclonal

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Clone: 206-2

Isotype: IgG2b

Product Details

Reactivity: Pig

Host: Mouse

Cross-Reactivity (Details): Species reactivity (tested):Porcine.Minimal Cross Reaction with Rat, Goat, Sheep, Donkey, Human (all <0.1 %) and Rabbit (0.1 %).

Purification: Affinity Chromatography

Immunogen: Porcine IgM.

Application Details

Application Notes: ELISA: 0.1 μg/mL (1/4000). Immunoprecipitation. Immunohistochemistry on Frozen Sections: 0.2 μg/mL (1/2000). Immunohistochemistry on Paraffin Sections (Does not react on routinely processedParaffin Section). Does not react in Western Blot with reduced and denatured samples.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.5 mL distilled water to make stock solution.

Concentration: 0.4 mg/mL

Buffer: PBS, pH 7.2 containing 5 mg/mL BSA as a stabilizer and 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated thawing and freezing.

Storage: 4 °C/-20 °C

Storage Comment: Store lyophilized product at 2-8 °C. Store stock solution or aliquots thereof at -28 °C.

Target Details

Target: IgM

Target Type: Antibody

Background: IgM antibodies form a major proportion of the primary antibody response, whereas the secondary response consists almost entirely of IgG. IgMs are often referred to as macroglobulins because of their high molecular weight. They form a pentameric structure consisting of five subunits and a single J-chain. Each subunit contains the well-known pairs of heavy and light chains, with the particular feature of the heavy (μ) chain containing one variable and four constant regions. The molecular weight of intact IgM is approximately 900 kDa. In polyacrylamide gel electrophoresis under denaturing conditions, the heavy chain migrates as a band of approximately 85 kDa.Synonyms: Pig Immunoglobulin M