Mouse anti-Human IgM Antibody (HRP)

Catalog No. ABIN457359

Quick Overview for Mouse anti-Human IgM Antibody (HRP) (ABIN457359)

Target: IgM

Clonality: Monoclonal

Conjugate: HRP

Application: ELISA, Western Blotting (WB)

Clone: CH2

Isotype: IgG1 kappa

Product Details

Reactivity: Human

Host: Mouse

Purpose: Anti-Hu IgM HRP

Specificity: The antibody CH2 reacts with Fc fragment of human IgM.

Cross-Reactivity (Details): Human

Purification: Purified antibody is conjugated with activated horseradish peroxidase (HRP) of high specific enzyme activity under optimum conditions and unconjugated antibody and free HRP are removed by size-exclusion chromatography.

Immunogen: Purified human IgM.

Application Details

Application Notes: Western blotting: Recommended dilution: 1 μg/mL.
Flow cytometry: Recommended dilution: 1-4 μg/mL, extracellular and intracellular staining.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 0.01 % thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.
Avoid prolonged exposure to light.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Target Details

Target: IgM

Target Type: Antibody

Background: Immunoglobulin M (IgM) is produced as a 900 kDa pentamer, which is an efficient complement binder. This antibody type is produced initially in the immune response and it is the first immunoglobulin class to be synthesized by a fetus or newborn. IgM antibodies do not cross the placenta. IgM concentration in blood is 0.12 g/l and its biological survival (plasma T1/2) is 5 days.,immunoglobulin M

Molecular Weight: 900 kDa