Rabbit anti-Cow IgG2 (Fc Region) Antibody

Catalog No. ABIN457581

Product Details

Target: IgG2

Binding Specificity: Fc Region

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Application: Immunodiffusion (ID), Immunoelectrophoresis (IEP)

Specificity: The reactivity of the antiserum is directed to the Fc subunit of subclass IgG2

Characteristics: Precipitating polyclonal rabbit antiserum to bovine IgG2, subclass specific
Total protein and IgG concentration in the antiserum are comparable to those of pooled normal rabbit serum. Antibody titre: Precipitin titre not less then 1:32 when tested against pooled normal bovine serum in agar block

Purification: Delipidated, heat inactivated, lyophilized whole antiserum.
Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. Special attention is given to the removal of antibodies to common Ig/Fab. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Immunogen: Pools of purified normal IgG2 isolated from pooled bovine serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG2 in bovine serum and other body fluids or to determine its concen-tration. To prepare an immunoadsorbent for the purification of bovine IgG2 from serum or plasma.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.

Buffer: No foreign proteins added.

Preservative: Without preservative

Handling Advice: Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4 °C, not refrozen, and preferably used the same day .

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Target Details

Target: IgG2

Abstract: IgG2 Products

Target Type: Antibody