Mouse anti-Human Bence-Jones Protein lambda (Chain lambda), (Free & Bound) Antibody

Catalog No. ABIN457819

Quick Overview for Mouse anti-Human Bence-Jones Protein lambda (Chain lambda), (Free & Bound) Antibody (ABIN457819)

Target: Bence-Jones Protein lambda

Clonality: Monoclonal

Application: ELISA, Western Blotting (WB)

Clone: NI 412 and NI 268

Isotype: IgG1 kappa

Product Details

Binding Specificity: Chain lambda, Free & Bound

Reactivity: Human

Host: Mouse

Characteristics: The reactivity of the this preparation of two monoclonal antibodies is restricted to polyclonal and monoclonal immunoglobulins of the lambda type, as well as free lambda light chains as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immuno-peroxidase staining. Purified monoclonal mouse antibody to human Ig lambda chain, free and bound

Purification: Purified

Immunogen: Highly purified Bence Jones lambda proteins isolated from pooled human urine.

Application Details

Application Notes: To identify the light chain type of immunoglobulins or free light chains in human serum, other body fluids, cell and tissue substrates and to determine its concentration in indirect techniques as ELISA, indirect immunoperoxidasestaining and immunoblotting.
The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:200 upwards, in Western blotting from 1:400 upwards. These data should be interpreted as general recommendations only.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized product by adding 0. 5 mL sterile distilled water.

Concentration: 0.4 mg/mL

Buffer: Purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline (pH 7.2).

Preservative: Without preservative

Handling Advice: Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4 °C, not refrozen, and preferably used the same day.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below - 24 °C.

Target Details

Target: Bence-Jones Protein lambda

Alternative Name: Bence Jones lambda