Mouse anti-Human IgM Antibody (TRITC)

Catalog No. ABIN457907

Quick Overview for Mouse anti-Human IgM Antibody (TRITC) (ABIN457907)

Target: IgM

Clonality: Monoclonal

Conjugate: TRITC

Application: Immunofluorescence (IF)

Clone: NI 179

Isotype: IgG1 kappa

Product Details

Reactivity: Human

Host: Mouse

Specificity: The reactivity of the antiserum is restricted to a isotype specific determinant of the IgM molecule as tested in indirect binding enzyme immunoassay, immunoblotting, immuno-precipitation and direct immunofluorescence staining of cytoplasmic Ig. Tetramethylrhodamine isothiocyanate-conjugated purified monoclonal mouse antibody to human IgM, isotype specific

Characteristics: Tetramethylrhodamine isothiocyanate-conjugated purified monoclonal mouse antibody to human IgM, isotype specific
Fluorochrome/IgG protein molar ratio (E/P) approximately 5.7.

Purification: Purified

Immunogen: Highly purified IgM isolated from human serum.

Application Details

Application Notes: To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as direct and indirect immunofluorescence staining of cytoplasmic IgM and cell membrane IgM.
The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:100. These data should be interpreted as general recommendations only.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized product by adding 0. 5 mL sterile distilled water.

Concentration: 0.4 mg/mL

Buffer: Purified monoclonal mouse IgG1 kappa conjugated with TRITC, lyophilized from a solution in phosphate buffered saline (pH 7.2).

Preservative: Without preservative

Handling Advice: Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Target Details

Target: IgM

Target Type: Antibody