Mouse anti-Human IgA Antibody

Catalog No. ABIN457921

Quick Overview for Mouse anti-Human IgA Antibody (ABIN457921)

Target: IgA

Clonality: Monoclonal

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Haemagglutination (H)

Clone: NI 69 (A89-034) and NI 184 (A89-035)

Isotype: IgG1 kappa

Product Details

Reactivity: Human

Host: Mouse

Specificity: The reactivity of the antiserum is restricted to determinants on the C H 2 domain of IgA. It reacts with both subclasses of IgA as tested in haemagglutination, haemagglutination inhibition, indirect binding enzyme immunoassay, competitive inhibition enzyme immunoassay, immunoblotting, immunoprecipitation, latex agglu-tination assay and histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148). Monoclonal mouse antiserum to human IgA, isotype specific

Purification: Delipidated, heat inactivated, lyophilized, stable whole ascites

Immunogen: Highly purified monoclonal IgA isolated from pooled human serum

Application Details

Application Notes: To identify the presence of IgA in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immuno-peroxidase and immunofluorescence staining of cytoplasmic IgA, haemagglutination and immunoblotting.
The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1000 upwards. These data should be interpreted as general recommendations only.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized ascites by adding 0. 5 mL sterile distilled water.

Concentration: 1 mg/mL

Buffer: No foreign proteins added.

Preservative: Without preservative

Handling Advice: Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Target Details

Target: IgA

Target Type: Antibody