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Mouse anti-Human IgG (Fc Region) Antibody

ELISA, WB, IF Monoclonal NI 335 and NI 343 IgG1 kappa unconjugated

Catalog No. ABIN457927

Datasheet as PDF

Target See all IgG products

IgG
Binding Specificity
2398

515

417

146

129

128

67

29

28

28

21

9

5

5

2

1

1
Fc Region
Reactivity
730

693

596

462

399

240

208

175

145

137

112

107

106

101

82

58

47

28

27

16

15

10

8

5

4

4

4

4

3

3

3

3

3

3

2

2

2

2

1

1

1

1
Human
Host
1762

1547

567

258

112

111

32

31

21

18

15

11

11
Mouse
Clonality
3391

179

16

1
Monoclonal
Application
2059

1985

1850

1805

998

920

896

487

424

234

234

149

98

84

65

56

51

30

28

25

20

15

11

9

8

7

5

5

4

4

4

3

2

2

1

1

1

1

1

1

1

1
ELISA, Western Blotting (WB), Immunofluorescence (IF)

Specificity

The reactivity of the antiserum is restricted to isotype specific determinants on the Fc part of the IgG molecule. The antiserum reacts with the 4 subclasses of IgG, as tested in indirect binding enzyme immunoassay, immunoblotting and indirect immuno-peroxidase staining of cytoplasmic Ig. Monoclonal mouse antiserum to human IgG, isotype specific

Purification

Delipidated, heat inactivated, lyophilized, stable whole ascites

Immunogen

Highly purified IgG isolated from pooled human serum

Clone

NI 335 and NI 343

Isotype

IgG1 kappa
Mouse anti-Human IgG (Fab Region) antibody
ELISA, WB, FACS Monoclonal 4A11 IgG2b unconjugated

Mouse anti-Human IgG (Fc Region) antibody (HRP)
ELISA, IHC, WB Monoclonal JDC-10 IgG1 HRP

Goat anti-Human IgG antibody (PE)
ELISA, FACS Polyclonal IgG PE F(ab')2 fragment

Mouse anti-Human IgG (Fc Region) antibody (APC)
ELISA, FACS Monoclonal H2 IgG2b APC

Goat anti-Human IgG (Heavy & Light Chain) antibody
ELISA, IHC, WB Polyclonal IgG unconjugated

Goat anti-Human IgG (Fc Region) antibody (Biotin)
ELISA, FLISA Polyclonal IgG Biotin

Rabbit anti-Human IgG (Heavy & Light Chain) antibody (Biotin)
ELISA, IHC, WB, FACS Polyclonal IgG Biotin

Goat anti-Human IgG (Heavy Chain) antibody (TRITC)
ELISA, FACS Polyclonal IgG TRITC

Goat anti-Human IgG (Heavy Chain) antibody (Texas Red (TR))
ELISA, FACS Polyclonal IgG Texas Red (TR)

Goat anti-Human IgG antibody (Biotin)
ELISA, FACS Polyclonal IgG Biotin F(ab')2 fragment

Application Notes

To identify the presence of IgG in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immuno-peroxidase and immunofluorescence staining and immunoblotting of cytoplasmic IgG.
The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.

Restrictions

For Research Use only
Format

Lyophilized

Reconstitution

Reconstitute the lyophilized ascites by adding 0. 5 mL sterile distilled water.

Concentration

1.0 mg/mL

Buffer

No foreign proteins added.

Handling Advice

Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.

Storage

4 °C/-20 °C

Storage Comment

The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
Target

IgG

Abstract

IgG Products

Target Type

Antibody