Mouse anti-Human IgG (Fc Region) Antibody

Catalog No. ABIN457927

Quick Overview for Mouse anti-Human IgG (Fc Region) Antibody (ABIN457927)

Target: IgG

Clonality: Monoclonal

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF)

Clone: NI 335 and NI 343

Isotype: IgG1 kappa

Product Details

Binding Specificity: Fc Region

Reactivity: Human

Host: Mouse

Specificity: The reactivity of the antiserum is restricted to isotype specific determinants on the Fc part of the IgG molecule. The antiserum reacts with the 4 subclasses of IgG, as tested in indirect binding enzyme immunoassay, immunoblotting and indirect immuno-peroxidase staining of cytoplasmic Ig. Monoclonal mouse antiserum to human IgG, isotype specific

Purification: Delipidated, heat inactivated, lyophilized, stable whole ascites

Immunogen: Highly purified IgG isolated from pooled human serum

Application Details

Application Notes: To identify the presence of IgG in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immuno-peroxidase and immunofluorescence staining and immunoblotting of cytoplasmic IgG.
The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized ascites by adding 0. 5 mL sterile distilled water.

Concentration: 1.0 mg/mL

Buffer: No foreign proteins added.

Handling Advice: Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Target Details

Target: IgG

Target Type: Antibody