Rabbit anti-Human IgA, IgG, IgM (Fab Region) Antibody

Catalog No. ABIN458091

Quick Overview for Rabbit anti-Human IgA, IgG, IgM (Fab Region) Antibody (ABIN458091)

Target: IgA, IgG, IgM

Clonality: Polyclonal

Application: Immunocytochemistry (ICC), Immunoelectrophoresis (IEP)

Isotype: IgG

Product Details

Binding Specificity: Fab Region

Reactivity: Human

Host: Rabbit

Specificity: The reactivity of the antiserum is directed to the major isotypes of the human immunoglobulin system (classes and both light chain types) including antibodies to common determinants, to class and to the surface determinants of the common Fab portion, as tested by immunoelectrophoresis and double radial immunodiffusion against pooled serum and purified immuno-globulins

Characteristics: Precipitating polyclonal rabbit antiserum to human immunoglobulins, IgG, IgA and IgM, heavy and light chains
Total protein and IgG concentration in the antiserum are comparable to those of pooled rabbit serum. Antibody titre: Precipitin titre not less than 1:64 when tested against normal human serum in agar block titration.

Purification: Delipidated, heat inactivated, lyophilized stable whole serum
Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibody activity with other serum protein. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Immunogen: Purified normal IgG and fractions containing IgA, and IgM isolated from human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Application Details

Application Notes: In precipitating techniques as immunoelectrophoresis, single and double radial immuno-diffusion (Mancini, Ouchterlony) to identify and measure immunoglobulins, as secondary antibody to precipitate the immunoglobulin in normal human serum. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such uses labelled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water.

Buffer: No foreign proteins added.

Preservative: Without preservative

Handling Advice: Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably used the same da y. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Target Details

Target: IgA, IgG, IgM

Alternative Name: IgG + IgA + IgM

Target Type: Antibody