Pig anti-Human kappa Light Chain (Free & Bound) Antibody (FITC)

Catalog No. ABIN458191

Quick Overview for Pig anti-Human kappa Light Chain (Free & Bound) Antibody (FITC) (ABIN458191)

Target: kappa Light Chain

Clonality: Polyclonal

Conjugate: FITC

Application: ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Isotype: IgG

Product Details

Binding Specificity: Free & Bound

Reactivity: Human

Host: Pig

Specificity: Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal Swine antiSerum to free and bound Human Ig kappa light chain.

Cross-Reactivity (Details): Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins and their fragments, since Ig of different species frequently share antigenic determinants. Crossreactivity of this antiSerum has not been tested in detail.

Characteristics: The reactivity of the antiserum is directed to the surface and hidden determinants of Ig kappa light chain. In immunoelectrophoresis this antiserum is reacting with polyclonal and monoclonal immunoglobulins of the kappa type, Bence Jones proteins as well as free light chains of the kappa type. This antiserum does not react with any other protein of human serum or plasma. This conjugate is intended for use in direct immunofluorescence for the detection of polyclonal immunoglobulins, purified monoclonal immunoglobulins of the kappa type as well as free light chains or Bence Jones proteins of the kappa type in serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Clone: Polyclonal

Purification: Purified

Immunogen: A pool of purified Bence Jones kappa proteins isolated from human urine. Freund's complete adjuvant is used in the first step of the immunization procedure.

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.Fluorochrome coupled purified swine IgG lyophilized from a solution in phosphate buffered saline ( pH 7.2). No preservative added, as it may interfere with the antibody activity. It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.

Buffer: Fluorochrome coupled purified swine IgG lyophilized from a solution in phosphate buffered saline ( pH 7.2). No preservative added, as it may interfere with the antibody activity.

Preservative: Without preservative

Storage: RT,4 °C,-20 °C

Storage Comment: The lyophilized product is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Target Details

Target: kappa Light Chain

Alternative Name: Ig kappa