Goat anti-Mouse IgG2b Antibody
ID, IEP, RID
Polyclonal
IgG
unconjugated
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- Target See all IgG2b products
- IgG2b
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Reactivity
- Mouse
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Host
- Goat
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Clonality
- Polyclonal
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Application
- Immunodiffusion (ID), Immunoelectrophoresis (IEP), Radial Immunodiffusion (RID)
- Specificity
- The reactivity of the antiserum is directed to the subclass IgG2b.
- Characteristics
- Total protein and IgG concentration in the antiserum are comparable to those of pooled normal goat serum. Antibody titre: Precipitin titre not les than 1:32 when tested against pooled normal mouse serum in agar block immunodiffu
- Purification
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Delipidated, heat inactivated, lyophilized, stable whole antiserum
Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. Special attention is given to the removal of antibodies to common Ig/Fab. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. - Immunogen
- Pools of purified homogenous IgG2b isolated from mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
- Isotype
- IgG
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- Application Notes
- In immunoelectrophoresis use 2 µL or equivalent against 120 µL antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 µL antiserum in a 3 mm diameter centre well and 2 µL serum samples (neat and diluted) in 2 mm diameter peripheral wells.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.
- Buffer
- No foreign proteins added.
- Preservative
- Without preservative
- Handling Advice
- Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4 °C, not ref rozen, and preferably used the same day.
- Storage
- 4 °C/-20 °C
- Storage Comment
- The lyophilized antiserum is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
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- Target
- IgG2b
- Abstract
- IgG2b Products
- Target Type
- Antibody
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