Rabbit anti-Mouse IgG1, IgG2a, IgG2b, IgG3 (Fc Region) Antibody

Catalog No. ABIN458430

Quick Overview for Rabbit anti-Mouse IgG1, IgG2a, IgG2b, IgG3 (Fc Region) Antibody (ABIN458430)

Target: IgG1, IgG2a, IgG2b, IgG3

Clonality: Polyclonal

Application: Immunoprecipitation (IP)

Product Details

Binding Specificity: Fc Region

Reactivity: Mouse

Host: Rabbit

Specificity: Precipitating polyclonal Rabbit antiSerum to Mouse IgG, Fc specific

Cross-Reactivity (Details): Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has been tested in double radial immunodiffusion anti several sera with the following results: cow - Cat - Chicken - Dog - Duck - Goat - Guinea Pig+ Human - Hamster + Horse - Monkey - pigeon - Rat + Sheep - Swine - Turkey - The absence of a reaction in double radial immunodiffusion does not exclude some reaction in more sensitive techniques.

Characteristics: The reactivity of the antiserum is directed to the Fc subunit of the IgG molecule. The antiserum contains antibodies to subclass-specific determinants as well as to determinants shared by two or more subclasses of IgG. In immunoelectrophoresis and double radial immunodiffusion, using various antiserum concentrations against normal mouse plasma, serum, and immunoglobulin fractions, no reaction is obtained with IgA, IgM and IgG/Fab fragments or any other serum protein In immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG, as secondary antibody to precipitate the immunoglobulin in normal mouse serum, to prepare an immunoadsorbent for the production of Ig-free mouse serum, plasma or non-Ig preparations.

Purification: Delipidated, heat inactivated, stable whole serum

Sterility: Heat-inactivated

Immunogen: Purified normal IgG, including all known subclasses, isolated from pooled mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Application Details

Application Notes: In immunoelectrophoresis use 2 μL or equivalent against 120 μL antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μL antiserum in a 3 mm diameter centre well and 2 μL serum samples (neat and diluted) in 2 mm diameter peripheral wells.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water.Delipidated, heat inactivated lyophilized stable whole serum No preservative added, as it may interfere with the antibody activity. No foreign protein added. Total protein and IgG concentration in the antiserum are comparable to those of pooled rabbit serum. No foreign proteins added. It is reconstituted by adding 1 mL sterile distilled water.

Buffer: Delipidated, heat inactivated lyophilized stable whole serum No preservative added, as it may interfere with the antibody activity. No foreign protein added. Total protein and IgG concentration in the antiserum are comparable to those of pooled rabbit serum.

Preservative: Without preservative

Storage: RT,4 °C,-20 °C

Storage Comment: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Target Details

Target: IgG1, IgG2a, IgG2b, IgG3

Alternative Name: IgG1 IgG2a IgG2b IgG3