Sheep anti-Human kappa Light Chain (Free & Bound) Antibody

Catalog No. ABIN458483

Quick Overview for Sheep anti-Human kappa Light Chain (Free & Bound) Antibody (ABIN458483)

Target: kappa Light Chain

Clonality: Polyclonal

Application: Immunoprecipitation (IP)

Product Details

Binding Specificity: Free & Bound

Reactivity: Human

Host: Sheep

Specificity: Precipitating polyclonal Sheep antiSerum to free and bound Mouse Ig kappa light chain.

Cross-Reactivity (Details): Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins and their fragments, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.

Characteristics: The reactivity of the antiserum is directed to the surface and hidden determinants of Ig kappa light chain. In immunoelectrophoresis this antiserum is reacting with polyclonal and monoclonal immunoglobulins of the kappa type, Bence Jones proteins as well as free light chains of the kappa type. This antiserum does not react with any other protein of mouse serum or plasma. To detect and identify the light chain type of immunoglobulins or free light chains in serum or other body fluids by immunoelectrophoresis or double radial immunodiffusion.
Clone: Polyclonal

Purification: Delipidated, heat inactivated lyophilized whole serum.

Sterility: Heat-inactivated

Immunogen: A pool of purified Bence Jones kappa proteins isolated from mouse urine. Freund's complete adjuvant is used in the first step of the immunization procedure.

Application Details

Application Notes: In immunoelectrophoresis use 2 μL serum or equivalent against 120 μL antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μL antiserum in 3 mm diameter center well and 2 μL serum samples (neat and serially diluted in 2 mm diameter peripheral wells. When carrying out analyses of Bence Jones proteins or free light chains in urine or serum using an immunodiffusion technique, special attention should be given to their faster rate of diffusion as compared to that of the antibody molecules in the antiserum. If they both start to diffuse into the gel in a double radial diffusion at the same time, an excess of the antigen may easily prevent visible precipitation. To prevent false negative results, the antiserum should be diffuse for about 2 hours before the antigen reservoir is filled.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles.Delipidated, heat inactivated lyophilized whole serum. No preservative added, as it may interfere with the antibody activity. It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles.

Buffer: Delipidated, heat inactivated lyophilized whole serum. No preservative added, as it may interfere with the antibody activity.

Preservative: Without preservative

Storage: RT,4 °C,-20 °C

Storage Comment: The lyophilized product is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Target Details

Target: kappa Light Chain

Alternative Name: Ig kappa