Rabbit anti-Pig IgA (Dimer) Antibody - Preadsorbed

Catalog No. ABIN458558

Product Details

Target: IgA

Binding Specificity: Dimer

Reactivity: Pig

Host: Rabbit

Clonality: Polyclonal

Application: Immunodiffusion (ID), Immunoelectrophoresis (IEP)

Specificity: The reactivity of the antiserum is restricted to the Fc part of the IgA molecule and the surface and hidden determinants of the secretory component. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against normal swine milk two precipitin lines may be obtained, one representing IgA and the other the free secretory component. Against normal swine serum only IgA is precipitated. No precipitation reaction is obtained with purified IgG, IgM, and IgG/Fab fragments

Cross-Reactivity (Details): The antiserum does not cross-react with any other component of the swine immunoglobulin system. Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. of this antiserum has not been tested in detail.

Characteristics: Precipitating polyclonal rabbit antiserum to swine secretory IgA
Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added. Antibody titre: Precipitin titre 1:32 when tested against pooled normal swine serum in agar-block immuno-diffusion titr
Delipidated, heat inactivated, lyophilized, stable whole antiserum

Purification: Preadsorption: Immunoaffinity adsorbed using insolubilized antigens

Immunogen: Highly purified secretory IgA isolated from pooled swine milk. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgA in swine milk or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of swine IgA from serum or exocrine secretions. Antisera to IgA or sIgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. This does not exclude the use of the antiserum in more sensitive techniques if proper controls are included.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C. Reconstitute the lyophilized antiserum by add ing 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4 °C, not refrozen, an d preferably used the same day.

Target Details

Target: IgA

Abstract: IgA Products

Target Type: Antibody