Goat anti-Rat IgG2c Antibody (FITC)

Catalog No. ABIN458703

Quick Overview for Goat anti-Rat IgG2c Antibody (FITC) (ABIN458703)

Target: IgG2c

Clonality: Polyclonal

Conjugate: FITC

Application: Immunofluorescence (IF)

Isotype: IgG

Product Details

Reactivity: Rat

Host: Goat

Specificity: The reactivity of the antiserum is directed to the subclass IgG2c as tested against rat sera and a panel of purified homogenous immunoglobulins.

Characteristics: Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal goat antiserum to rat IgG2c, subclass specific
Fluorochrome/IgG protein molar ratio (F/P) is approximately 4.5.

Purification: Undesired traces of antibody activity are eliminated by immunoaffinity chromatography as required. Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography.

Immunogen: Pools of highly purified homogenous IgG2c isolated from rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Application Details

Application Notes: To identify and measure IgG2c, antigen or antibody, at the cellular and subcellular level by immuno-fluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen or immune complex using a reference antibody of rat origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted.
The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80.

Comment:

Excitation/Emission wavelength: 494 nm/514 nm

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.

Concentration: 10 mg/mL

Buffer: Fluorochrome-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).

Preservative: Without preservative

Handling Advice: Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized conjugate is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Target Details

Target: IgG2c

Target Type: Antibody