Rabbit anti-Rat IgG (Heavy & Light Chain) Antibody

Catalog No. ABIN458709

Quick Overview for Rabbit anti-Rat IgG (Heavy & Light Chain) Antibody (ABIN458709)

Target: IgG

Clonality: Polyclonal

Application: Immunocytochemistry (ICC), Immunoelectrophoresis (IEP)

Isotype: IgG

Product Details

Binding Specificity: Heavy & Light Chain

Reactivity: Rat

Host: Rabbit

Specificity: The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion.

Characteristics: Total protein and IgG concentration in the antiserum are comparable to those of pooled rabbit serum. Antibody titre: Precipitin titre not less than 1:128 when tested against normal rat serum in agar block titration.

Purification: Delipidated, heat inactivated lyophilized stable whole serum
No adsorption required.

Immunogen: Purified normal IgG, including all known subclasses, isolated from pooled rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Application Details

Application Notes: In precipitating techniques as immunoelectrophoresis, single and double radial immuno-diffusion (Mancini, Ouchterlony) to identify the presence of IgG and to determine its concen-tration. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such use labelled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water.

Buffer: No foreign proteins added.

Preservative: Without preservative

Handling Advice: Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably u sed the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Target Details

Target: IgG

Target Type: Antibody