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Mouse anti-Human Ig (Chain lambda), (Light Chain) Antibody (FITC)

FACS Monoclonal 4C2 IgG1 FITC
Catalog No. ABIN487443

Quick Overview for Mouse anti-Human Ig (Chain lambda), (Light Chain) Antibody (FITC) (ABIN487443)

Target

Ig

Clonality

  • 123
  • 94
Monoclonal

Conjugate

  • 54
  • 25
  • 23
  • 21
  • 18
  • 14
  • 12
  • 10
  • 5
  • 4
  • 4
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
FITC

Application

  • 173
  • 137
  • 101
  • 69
  • 57
  • 26
  • 22
  • 12
  • 12
  • 7
  • 7
  • 6
  • 4
  • 4
  • 2
  • 1
  • 1
  • 1
Flow Cytometry (FACS)

Clone

4C2

Isotype

IgG1
  • Binding Specificity

    • 80
    • 77
    • 62
    • 38
    • 4
    • 2
    • 1
    Chain lambda, Light Chain

    Reactivity

    • 117
    • 63
    • 20
    • 18
    • 9
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human

    Host

    • 102
    • 76
    • 39
    • 18
    • 3
    • 1
    • 1
    Mouse

    No Cross-Reactivity

    Hamster, Sheep (Ovine), Goat, Rabbit, Guinea Pig

    Cross-Reactivity (Details)

    Species reactivity (tested):Human.
  • Application Notes

    Flow cytometry analysis of human blood cells using 20 μL reagent / 100 μL of whole bloodor 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Buffer

    Phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C

    Storage Comment

    Store the antibody at 2 - 8 °C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
    Shelf life: one year from despatch.

    Expiry Date

    12 months
  • Target

    Ig

    Alternative Name

    Immunoglobulin

    Background

    Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda, both having a molecular weight of 22.5 kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.
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