Mouse anti-Human Ig (Chain lambda), (Light Chain) Antibody

Catalog No. ABIN487444

Quick Overview for Mouse anti-Human Ig (Chain lambda), (Light Chain) Antibody (ABIN487444)

Target: Ig

Clonality: Monoclonal

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 4C2

Isotype: IgG1

Product Details

Binding Specificity: Chain lambda, Light Chain

Reactivity: Human

Host: Mouse

Cross-Reactivity (Details): Species reactivity (tested):Human. Hamster, Sheep, Goat, Rabbit, Guinea pig.

Purification: Protein A affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Flow cytometry. Positive control: RAMOS human cell line. Immunohistochemistry on frozen and paraffin sections. ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeatedfreezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details

Target: Ig

Alternative Name: Immunoglobulin

Background: Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda, both having a molecular weight of 22.5 kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.