Recombinant Rabbit anti-Human IgG Heavy Chain (LOC396781) Antibody

Catalog No. ABIN4955830

Quick Overview for Recombinant Rabbit anti-Human IgG Heavy Chain (LOC396781) Antibody (ABIN4955830)

Target: IgG Heavy Chain (LOC396781)

Antibody Type: Recombinant Antibody

Clonality: Monoclonal

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: IG1707R

Isotype: IgG kappa

Product Details

Reactivity: Human

Host: Rabbit

Purification: Protein A affinity chromatography

Immunogen: Human Ig Gamma Chain was used as the immunogen for this recombinant IgG antibody.

Application Details

Application Notes: The optimal dilution of the recombinant IgG antibody for each application should be determined by the researcher.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,

Restrictions: For Research Use only

Handling

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant IgG antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details

Target: IgG Heavy Chain (LOC396781)

Alternative Name: IgG Heavy Chain

Background: Recognizes a protein of 75 kDa, identified as gamma heavy chain of human immunoglobulins. It reacts with all sub-classes of gamma chain of human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This mAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.