Sheep anti-Guinea Pig IgG1 (Subclass specific) Antibody

Catalog No. ABIN5540420

Quick Overview for Sheep anti-Guinea Pig IgG1 (Subclass specific) Antibody (ABIN5540420)

Target: IgG1

Clonality: Polyclonal

Application: Immunoprecipitation (IP), Immunodiffusion (ID)

Product Details

Binding Specificity: Subclass specific

Reactivity: Guinea Pig

Host: Sheep

Purification: Serum

Immunogen: Highly purified normal IgG1 isolated from pooled guinea pig serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Application Details

Application Notes: Immunoprecipitation. Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG1 in guinea pig serum and other body fluids or to determine its concentration. Directions for Use : Immunoelectrophoresis: Use 2 μL or equivalent against 120 μL antiserum. Double Radial Immunodiffusion use a rosette arrangement with 10 μL antiserum in a 3 mm diameter centre well and 2 μL serum samples (neat and diluted) in 2 mm diameter peripheral wells. Antibody titre: Precipitin titre not less than 1/16 when tested against normal guinea pig serum in agar block titration.

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details

Target: IgG1

Target Type: Antibody