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Goat anti-Human lambda Light Chain (Lambda-IgLC) Antibody

Rockland 709-1111 Lambda-IgLC WB, IHC, ELISA Polyclonal IgG unconjugated F(ab')2 fragment
Rockland
Catalog No. ABIN5608051
Supplier Product No.: 709-1111
  • Target See all Lambda-IgLC products
    Lambda-IgLC (lambda Light Chain (Lambda-IgLC))
    Fragment
    F(ab')2 fragment
    Reactivity
    • 31
    • 11
    Human
    Host
    • 20
    • 12
    • 9
    • 1
    Goat
    Clonality
    • 29
    • 13
    Polyclonal
    Application
    • 27
    • 22
    • 21
    • 20
    • 10
    • 10
    • 9
    • 9
    • 7
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), ELISA
    Supplier Product No.
    709-1111
    Supplier
    Rockland
    Purpose
    F(ab')2 Human (lambda chain) Antibody
    Cross-Reactivity (Details)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgG and Human Serum.  No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).  Specificity was confirmed by ELISA at less than 1 % cross reactivity against other human heavy or light chain isotypes.
    Characteristics
    Anti-Human IgG (kappa chain) antibody generated in goat detects specifically human IgG (kappa chain). This secondary antibody anti-Human is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
    Purification
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
    Sterility
    Sterile filtered
    Immunogen
    Optional[Immunogen]: Human lambda light chain
    Isotype
    IgG
  • Application Notes
    Application Note: Suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:1,000 - 1:5,000 ELISA Dilution: 1:6,000 Other: User Optimized
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0

    Stabilizer: None

    , Preservative:0.01 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 
    Expiry Date
    12 months
  • Target
    Lambda-IgLC (lambda Light Chain (Lambda-IgLC))
    Alternative Name
    lambda light chain (Lambda-IgLC Products)
    Background
    Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate into tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
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