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Goat anti-Human kappa Light Chain Antibody (Biotin)

Rockland 609-1610 WB, ELISA, IHC Polyclonal IgG Biotin
Rockland
Catalog No. ABIN5608052
Supplier Product No.: 609-1610
  • Target See all kappa Light Chain products
    kappa Light Chain
    Reactivity
    • 43
    • 17
    Human
    Host
    • 21
    • 16
    • 15
    • 4
    • 3
    • 1
    Goat
    Clonality
    • 42
    • 18
    Polyclonal
    Conjugate
    • 14
    • 9
    • 7
    • 7
    • 7
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Biotin
    Application
    • 36
    • 34
    • 23
    • 18
    • 16
    • 14
    • 10
    • 9
    • 9
    • 8
    • 8
    • 6
    • 5
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
    Supplier Product No.
    609-1610
    Supplier
    Rockland
    Purpose
    Human (kappa chain) Antibody Biotin Conjugated
    Cross-Reactivity (Details)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-Goat Serum.  Specificity was confirmed by ELISA minimal against other human heavy or light chain proteins.
    Characteristics
    Anti-Human IgM (mu heavy chain) Biotin generated in goat detects specifically Human IgM mu heavy chain.
    Purification
    Anti-Human k was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
    Immunogen
    Optional[Immunogen]: Human kappa light chain
    Isotype
    IgG
  • Application Notes
    Application Note: Anti-Human K Biotin Conjugated Antibody has been tested by ELISA and Western Blot and is suitable for ELISA, Western Blot and Immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:200,000 Other: User Optimized
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    , Preservative:0.01 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    Expiry Date
    12 months
  • Target
    kappa Light Chain
    Abstract
    kappa Light Chain Products
    Background
    The anti-Human κ (kappa chain) Antibody detects the kappa chain subunit. Immunoglobulins are heterotetramers composed of 2 immunoglobulin heavy and 2 immunoglobulin light chains. The immunoglobulin light chain is the small polypeptide subunit of an antibody (immunoglobulin). In humans the light chains can be categorized into kappa type or lambda type and both are used to construct the antigen binding F(ab) region of an antibody along with the variable region of the heavy chain. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Human IgG kappa is conjugated to biotin.
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