Goat anti-Ferret IgA, IgG, IgM Antibody (Alkaline Phosphatase (AP))

Catalog No. ABIN5608082

Supplier Product No.: 618-105-130

Quick Overview for Goat anti-Ferret IgA, IgG, IgM Antibody (Alkaline Phosphatase (AP)) (ABIN5608082)

Target: IgA, IgG, IgM

Clonality: Polyclonal

Conjugate: Alkaline Phosphatase (AP)

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Product Details

Reactivity: Ferret

Host: Goat

Supplier Product No.: 618-105-130

Supplier: Rockland

Purpose: Ferret IgG IgA IgM (H&L) Antibody Alkaline Phosphatase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Goat Serum.  This reagent is suitable for the detection of all Ferret immunoglobulin isotypes, subclasses and chain combinations.

Characteristics: Anti-Ferret IgG Alkaline Phosphatase antibody generated in goat detects ferret IgG.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Ferret antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Sterility: Sterile filtered

Immunogen: Optional[Immunogen]: Ferret IgG IgA and IgM whole molecules

Application Details

Application Notes: Application Note: Anti-Ferret IgG IgA IgM Alk Phos conjugate is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:500 - 1:3,000 ELISA Dilution: 1:2,000 - 1:10,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Expiry Date: 12 months

Target Details

Target: IgA, IgG, IgM

Alternative Name: IgG,IgA,IgM

Target Type: Antibody

Background: Anti-Ferret IgG IgA IgM Alkaline Phosphatase Antibody generated in goat detects immunoglobulin G, A, and M from ferret. Immunoglobulin G binds to antigens and can neutralize or opsonize targets, and are predominantly involved in the secondary immune response. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Immunoglobulin M, or IgM, is a pentamer composed of 5 immunoglobulin molecules linked through their F(c) domain by the J chain.