Goat anti-Human lambda Light Chain (Lambda-IgLC) Antibody (FITC)

Catalog No. ABIN5608120

Supplier Product No.: 709-1211

Product Details

Target: Lambda-IgLC (lambda Light Chain (Lambda-IgLC))

Fragment: F(ab')2 fragment

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: FITC

Application: Flow Cytometry (FACS), Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Supplier Product No.: 709-1211

Supplier: Rockland

Purpose: F(ab')2 Human (lambda chain) Antibody Fluorescein Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Goat Serum.  No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).   Specificity was confirmed by ELISA at less than 1 % cross reactivity against other human heavy or light chain isotypes.

Characteristics: F(ab')2 HUMAN kappa Antibody Fluorescein Conjugated is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Immunogen: Optional[Immunogen]: Human lambda light chain

Isotype: IgG

Application Details

Application Notes: Application Note: F(ab')2 Anti-Human (lambda light chain) Fluorescein Conjugated Antibody has been tested by western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Flow Cytometry Dilution: 1:500 - 1:2,500 FLISA Dilution: 1:10,000 - 1:50,000 IF Microscopy Dilution: 1:1,000 - 1:5,000 Other: FLOW CYTOMETRY 1:500 - 1:2,500

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details

Target: Lambda-IgLC (lambda Light Chain (Lambda-IgLC))

Alternative Name: lambda light chain (Lambda-IgLC Products)

Background: F(ab')2 Anti-Human (lambda light chain) Antibody generated in goat detects human lambda light chain. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunofluorescence, IHC, and other immunoassays. This F(ab')2 Anti-Human (lambda light chain) Antibody is conjugated to fluorescein.