Goat anti-Human lambda Light Chain (Lambda-IgLC) Antibody (HRP)

Catalog No. ABIN5608130

Supplier Product No.: 709-1311

Product Details

Target: Lambda-IgLC (lambda Light Chain (Lambda-IgLC))

Fragment: F(ab')2 fragment

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: HRP

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Supplier Product No.: 709-1311

Supplier: Rockland

Purpose: F(ab')2 Human (lambda chain) Antibody Peroxidase Conjugated)

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum.  No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).   Specificity was confirmed by ELISA at less than 1 % cross reactivity against other human heavy or light chain isotypes.

Characteristics: F(ab')2 anti-Human (kappa chain) antibody generated in goat detects specifically Human IgG kappa chain. This secondary antibody anti-Human is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays. F(ab')2 Anti-Human (kappa chain) Antibody is ideal for investigators in Immunology, Cancer, and Microbiology research.

Purification: F(ab')2 Human lambda chain Antibody Peroxidase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Human antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Immunogen: Optional[Immunogen]: Human lambda light chain

Isotype: IgG

Application Details

Application Notes: Application Note: F(ab')2 Anti-Human lambda chain Peroxidase Conjugated Antibody has been tested by ELISA and western blot and is ideal for ELISA, western blotting, Immunohistochemistry, as well as other antibody detection methods. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:5,000 ELISA Dilution: 1:10,000 - 1:50,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details

Target: Lambda-IgLC (lambda Light Chain (Lambda-IgLC))

Alternative Name: lambda light chain (Lambda-IgLC Products)

Background: F(ab')2 Anti-Human lambda chain Antibody generated in goat detects lambda light chain from human. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays. F(ab')2 Anti-Human lambda chain Antibody is conjugated to Peroxidase.